Hi Bill,
yes using grounds which are more than 48 hours old (and even more than 24 hours old during the warmer summer months) will defintiely increase your instances of green mould.
I’m not sure on the effectiveness of pasteurising grounds by microwave – my feeling is that if mould has already begun to develop (this may be hard to see at first) then it would probably not be a very effective way to kill it off. Only a full sterilisation at 121C would guarantee killing it.
With regards pasteurising straw – the best method is cold water pasteursing in a lime water bath, documented in the additional resources of module 3 inside the course.
Hope this helps.
Best wishes, Adam